汉族成人牙周炎患者白细胞介素-1基因多态性分析

Analysis of the IL-1 Gene Polymorphism in Han Nationalities with Adult Periodontitis

【目的】分析汉族成人牙周炎患者的白细胞介素 1(IL 1)基因家族多态性特征。【方法】将 10 9名汉族成人牙周炎患者分为轻、中、重度三组 ,用PCR和限制性酶切方法 ,对其与 2 0名正常人的IL 1A 889基因位点 ,IL 1B +395 3基因位点、IL 1RN内含子 2进行多态性分析。【结果】重度成人牙周炎患者的IL 1B等位基因 2携带率 (35 9% )较正常人 (15 0 % )和轻 (6 8% )、中度 (18 8% )牙周炎患者的高 ,其同时携带IL 1A等位基因 2、IL 1B等位基因 2的发生率亦比其他各组患者的高(P<0 0 5 )。而IL 1A、IL 1RN的多态性分布在各组中无明显差异。【结论】汉族重度成人牙周炎患者与轻度、中度患者的IL 1B(+395 3)基因多态性差异可能是他们临床表现差异的内在原因 ,这有助于提高对成人牙周炎疾病本质的认识。

To analyze the characteristic of the IL 1 gene polymorphism in Han nationality with adult periodontitis. 109 Han adults with periodontitis (include mild, moderate, severe group) and 20 periodontally healthy controls were enrolled. PCR and restriction enzymes were used to identify specific polymorphisms, and the frequency of polymorphisms in the IL 1A gene at position 889?IL 1B gene at position +3953 and IL 1RN intron 2 were investigated. The IL 1B allele 2 carriage rate (35 9%) in severe periodontitis was higher than that in healthy(15 0%)?mild(6 8%) and moderate(18 8%) groups, the composite genotype (IL 1A allele 2 plus IL 1B allele 2) occurrence rate in severe patients was also higher than that in the other groups (P<0 05). The frequency of IL 1A( 889) and IL 1RN(intron 2) polymorphisms was not statistically significant among groups. [Conclusion] The difference in IL 1B gene polymorphism between the severe patients and the other groups may be the most important intrinsic factor attribute to the differences in clinical manefestation. It may provide useful informations for recognizing the essence of periodontitis.