人膀胱癌体外药物敏感性预测与临床应用

Drug Susceptibility Trial in Vitro and Clinical Application in Human Bladder Neoplasms

目的:探讨人膀胱癌体外药敏试验在膀胱癌术后选药预防复发中的作用。方法:药物选用MMC、ADMHPT、BCG、榄香稀乳。采用MTT法分别计算5种药物对人膀胱癌细胞的抑制率。并选择抑制率最高的药物应用于临床。结果:所选5种药物对膀胱癌均有敏感性。但榄香稀乳始终显示出对癌细胞的抑制率最高,并获得临床证实。另外,在所选药物中抑制率与药物浓度呈正相关。结论:人膀胱癌体外药物敏感性研究为膀胱癌术后灌注化疗的选药提供了一种更有针对性的方法。

Objective:To study the effect of drug susceptibility trial in vitro in human bladder neoplasms for preventing the recurrence of bladder tumor after operation.Methods:The sensitivity rate of MMC,ADM,HPT,BCG and Elemene emulsion for inhibiting human bladder cancer cells were detected by MTT method,and the most sensitive drug was used clinically.Results:All of the above mentioned medicines could effectively inhibit the bladder cancer cells,but among them elemene is the most sensitive one,and the growth inhibition rate was directly correlated with the concentration.Conclusion:The drug susceptiblity trial provides an approach to aim directly in selecting medicine for intravesical instillation in preventing the recurrence of bladder cancer after operation.