β-榄香烯对胃癌SGC-7901细胞c-myc基因表达及端粒酶活性的影响

Effects of B-Elemene on Both Telomerase Activity and Expression of c-myc gene of Gastric Cancer SGC-7901Cell

目的:探讨β-榄香烯对胃癌SGC-7901细胞c-myc基因表达及端粒酶活性的影响。方法:应用β-榄香烯处理人胃癌细胞系SGC-7901后,采用RT-PCR检测c-myc基因mRNA的变化,采用流式细胞仪免疫荧光技术检测c-myc蛋白表达,用端粒酶PCR-ELISA法检测端粒酶活性。结果:β-榄香烯可下调c-myc基因表达及抑制端粒酶活性。结论:β-榄香烯可抑制胃癌细胞端粒酶活性,可能与下调c-myc基因表达有关。

Objective:To study the effects of B-elemene on both telomerase activity and expression of c-myc gene.Methods:After gastric cancer SGC-7901cells were treated by B-elemene,we studied the expression of mRNA of c-myc gene using RT-PCR,and the expression of c-myc protein using flow cytometry immunoflurescence technique and telomerase activity using telomerase PCR-ELISA assay.Results:B-elemene could reduce the expression of c-myc gene and repress the telomerase activity of gastric cancer SGC-7901cells.Conclusion:B-elemene can repress the telomerase activity of gastric cancer cells.The repression would be related to the decline of expression of c-myc gene.