TIMP-1特异性核酶的计算机设计

Computer-assisted design of TIMP-1 ribozyme

目的 :设计特异性切割 TIMP- 1m RNA的核酶 ,并嵌入 U6sn RNA中以提高其稳定性。方法 :根据 Sym ons的“锤头结构”,计算机辅助确定最佳切点。 结果 :针对第 12 3、2 99和 35 3位设计了 3个核酶 ,嵌入 U6sn RNA后 ,结合能量变化不大。结论 :计算机辅助设计是研究核酶过程中不可或缺的重要步骤 ;嵌入 U6sn

Objective: To design the ribozymes to cleave human TIMP 1 mRNA, and embed them into U 6snRNA to make them stable. Methods: Ribozymes were designed according to the “hammerhead structure” described by Symons.Computer was used to analyze the possible cleavage sites. Results: Three ribozymes targeting the nt123, nt299 and nt353 on TIMP 1 mRNA were designed. Embedding ribozyme in U 6snRNA had little effect on its binding with the substrate. Conclusion: Computer assisted design is indispensable in studying ribozyme. Embedding ribozymes in U 6snRNA may be a good way to solve the problems existing in ribozyme study. [