内毒素脂多糖对培养肾小管上皮细胞凋亡和增殖的影响

Effects of lipopolysaccharide on apotosis and proliferation of cultured tubular epithelial cells

目的 观察肾小管上皮细胞 (Tubularepitheliacell,TEC)在含内毒素脂多糖 (Lipopolysaccharide ,LPS)微环境中细胞凋亡和功能改变 ,探讨LPS直接损害肾小管的机制。方法 在含内毒素LPS的介质中体外培养肾小管上皮细胞 ,检测不同时相细胞凋亡、增殖能力及分泌肿瘤坏死因子 α(Tumornecrosisfactorα ,TNF α)能力的变化。结果 LPS有明显的抑制TEC增殖 ,促进TEC分泌TNF α ,且随着LPS浓度的增大 ,其作用更为明显 ;LPS还具有明显促细胞凋亡作用 ,呈剂量依赖性 ;相关分析表明 ,细胞凋亡与LPS促TEC分泌TNF α的量有明显正相关。结论 LPS可抑制TEC增殖 ,促进细胞凋亡 ,可能是通过TNF α而起作用 ,这可能是LPS导致脓毒症性肾衰或急性间质性肾炎小管间质损害的机制之一。

Objective To explore the effects of lipopolysaccharide(LPS) in culture medium on the apotosis and function of cultured tubular epithelial cells (TEC). Methods Purified human TECs were cultured in the medium containing LPS of different concentration. The apotosis, prolifera tive ability and production of TNF α were determined at various stages of culture. Results LPS restrained the proliferative ability of TEC obviously and enhanced the apotosis and the production of TNF α in a dose dependent manner. The percentage of apotosis had a positive correlation with the production of TNF α by TEC. Conclusion The inhibition of TEC proliferation and the enhancement of apoptosis induced by LPS might depend on TNF α, which may be one of the mechanisms of LPS inducing tubulo interstitial damage in acute renal failure or acute tubulo interstitial nephritis.