类似先天性静止性夜盲症视觉电生理异常大鼠1例

A rat with retinal hereditary disease similar to congenital stationary night blindness patient's ERG

目的 观察正常 SD大鼠和一例患遗传性视网膜疾病大鼠的视觉电生理学特点 .方法 按国际临床视觉电生理学会标准化方案 ,采用 REPIport系统和自制的氯化银角膜电极、不锈钢针状电极对成年雄性 SD大鼠进行视网膜电图暗适应 (ERG)、振荡电位 (OPs波 )和明适应 ERG进行观察 .结果 正常大鼠 ERG特性与人类相似 ,一只大鼠视觉电生理明显异常 ,ERG呈负波型 ,表现为暗适应 ERG b波基本消失 ,暗适应 ERG a波幅值明显降低 ,明适应 ERG a,b波幅值降低 ,峰时延长 ,OPs波幅值基本消失 .该例大鼠的视觉电生理学特点与人类先天性静止性夜盲症患者视觉电生理特点相似 ,而与视网膜色素变性病例不同 .结论 发现一例自然发生的基因突变大鼠 。

AIM To compare the characteristic of the visual electrophysiology between normal SD rats and a case of abnormal rat with retinal hereditary disease. METHODS According to the International Clinic Visual ElectrophysiologicalStandard Program, 22 adult male SD rats were tested to observe the scotopic retinoelectrogram (Scot. ERG), photopic retinoelectrogram (Phot. ERG) and oscillatory potentials (OPs) with RETIport system, Ag AgCl corneal contact electrode and stainless steel needle electrode. RESULTS The ERGs and OPs waves of the normal 21 rats were similar to that of human. However, one of the case (both eyes) was abnormal. ERGs and OPs. The Scot. ERGs showed a negative wave, in which the b wave almost disappeared and the amplitude of a wave obviously descended. The amplitude of a, b wave of Phot. ERGs declined, the letancy delayed. The amplitude of the OPs almost disappeared. The electrophysiology character of the rat was similar to the human Congenital Stationary Night Blindness, but very different from retinal pigmentosa. CONCLUSION One case of congenital gene mutation rat are found. The clinical visual electrophysiology test shows that the rat had inherited retinal degeneration disease.