山东地区汉族人HLA-DPB1基因单核苷酸多态性

A preliminary study of chines HLA-DPB1 gene

使用第十一届国际组织相容性会议提供的HLA DPB1引物序列 ,由PCR技术扩增人HLA DPB1基因第二外显子。产物纯化后 ,直接核酸序列分折确定个体的HLA DPB1外显子核酸序列。使用基因定型软件与由国际上公布的HLA DPB1核酸序列构建的基因型核酸序列数据库进行比较 ,确定个体的基因型别、确定等位基因类型。研究结果提示中国人HLA DPB1第二外显子基因序列与国际上公布的序列基本一致 ,但有不同之处 ,多处存在单核苷酸多态性 (SNP) ,提示存在有新的等位基因。对 51例个体研究显示 ,出现频率最高的等位基因是DPB1 0 2 0 1 2。

Using HLA DPB1 primer sequence provided by the eleventh International Histocompability Conference, human HLA DPB1 gene exon 2 was amplified by PCR. HLA DPB1 exon nucleic acid sequence was determined by direct nucleic acid sequence analysis of PCR product after purification. The results were compared with genotype nucleic acid sequence database published by gene typing software to determine the individual genotype and allele. The results showed that there was difference between Chinese HLA DPB1 exon 2 gene sequence and the sequence published. Mononucleic acid polymorphism existed in many parts and suggested that there was a new allele. Among the 51 cases studied, DPB1*02012 has the highest frequency.