摘要
为了解支气管哮喘后豚鼠大脑和肺组织c fos基因表达的变化 ,探讨c fos表达在豚鼠哮喘发病中的可能意义。我们复制卵蛋白致敏哮喘豚鼠模型 ,采用免疫组织化学ABC方法 ,对Fos蛋白在大脑和肺脏内的分布情况进行观察。结果发现 :哮喘组豚鼠大脑和肺内c fos表达较对照组明显增加 ,其Fos阳性产物在大脑主要分布于额顶皮质、边缘前脑 (扣带皮质、梨状皮质和中央杏仁核等 )、丘脑室旁核、下丘脑室旁核、视上核、下丘脑外侧区、下丘脑室周核、孤束核、最后区和延髓腹外侧区内 ,小脑内无明显Fos分布密集区。原癌基因c fos的表达增强在豚鼠支气管哮喘发病过程中可能起一定作用。
To study the significance and distribution of c fos expression in brain and lung tissue of ovalbumin induced acute allergic asthmatic guinea pigs, Fos proteins were observed in asthmatic guinea pigs induced by sensitizing and challenging with albumin by immunohistochemical ABC method. The results showed that the quantity of Fos positive neurons of asthma group increased markedly in the brain and lung tissue comparing with normal group. Fos positive products were mainly concentrated in frontal and parietal cortex, forebrain limbic system (cingulate cortex, piriform cortex and central amygdaloid nucleus), paraventricular thalamic nucleus, hypothalamic paraventricular nucleus, supraoptic nucleus, lateral hypothalamic area, periventricular hypothalamic nucleus, nucleus of solitary tract, area postrema and ventrolateral medulla. No distinct accumulation of Fos proteins were found in the cerebellum. It is suggested that the increase in proto oncogene expression of c fos might be involved in the attack of asthmatic guinea pigs.
出处
《基础医学与临床》
CSCD
北大核心
2002年第1期63-66,共4页
Basic and Clinical Medicine
