HPLC法测定虫草精王浆胶囊中10-羟基-2-癸烯酸的含量

Determination of 10-Hydroxy-2-decenoic acid in Chongcaojing Wangjiang Capsule by HPLC

[目的 ]采用高效液相色谱法测定虫草精王浆胶囊中 1 0 -羟基 -2 -癸烯酸的含量。 [方法 ]色谱柱 :DiscoveryC18;检测波长 :2 0 9nm ;流动相 :乙腈 -0 .5 %磷酸溶液 ( 2 5∶75 ) ;流速 :1 .0mL/min ;柱温 :2 5℃。 [结果 ]线性范围在 0 .0 2 1 4 4～ 0 .385 92 μg范围内 ,r=1 ,平均加样回收率为 1 0 1 .8% ,RSD =1 .6 % (n =5 )。 [结论 ]该法可作为虫草精王浆胶囊的质量控制标准。

To determine the content of 10-Hydroxy -2-decenoic acid in Chongcaojing Wangjiang Capsule by HPLC.The column was Discovery C 18 .The detective wavelength was at 209 nm. The mobile phase was acetonitrile: 0.5% phosphoric acid (25∶75) with the flow rate being 1.0 mL/min and the column temperature at 25℃.There was a good linearity (r=1) winthin the range of 0.02144 ～ 0.38592 μg. The average recovery rate was 101.8%, RSD= 1.6%. [Conclusion] This method can be used for the quality control standard of Chongcaojing Wangjiang Capsule.