米非司酮对早孕妇女血中NO和cGMP及绒毛组织中NOS的影响

Effects of mifepristone on the levels of serum NO and plasma cGMP and the content of villi NOS in early-pregnant women.

目的:探讨米非司酮对早孕妇女血中 NO和 cGMP的影响及米非司酮流产后出血时间延长与血中 NO和 cGMP关系。方法:采用硝酸酶还原法、放射免疫法和一氧化氮合酶活性测定法测定米非司酮用药前、后和流产第 15天血中 NO、cGMP浓度以及米非司酮流产、人工流产后绒毛中 NOS的含量。结果:服米非司酮前早孕组血清NO浓度显著高于正常未孕组(P<0.01),服药后显著低于服药前(P<0.01),米非司酮流产后出血时间≥15天组血清NO显著高于出血时间<15天组(P<0.01)。血浆 cGMP浓度与血清 NO呈显著正相关(r=0.82,P<0.01)。米非司酮流产后绒毛中 NOS含量显著高于人工流产组(P<0.01)。结论:米非司酮使早孕妇女血中NO-cGMP降调,米非司酮流产后出血时间延长可能与 NO-cGMP有关。

Objective:To study the relationship between the mechanism of terminating early pregnancy and pro-longed bleeding after mifepristone abortion and the changes of nitric oxide (NO) and cyclic guanosine monophosphate(cGMP) levels in the blood. NO and cGMP in blood and nitric oxide synthase(NOS) in chorionic villi were determined by NO3-/NO2- method, radioimmunoassay and NOS activity method respectively befor and after the treatment. The concentration of serum NO increased signifi-cantly in early pregnant women(P<0.01) ,compared to that of nonpregnant group and decreased evi-dently after administration of mifepristone (P<0.01); after medical abortion, it was higher in pa-tients with prolonged bleeding for≥15days than that for<15days(P<0.01). Concentration of plas-ma cGMP and that of serum NO were positively correlated (r=0.82,P<0.01).The level of NOS in chorionic villi of medical abortion was significantly higher than that of surgical abortion (P<0.01). NO、cGMP decreased after mifepristone abortion and the cause of prolongation of bleeding after medi-cal abortion is likely related to NO-cGMP system.