瑞香狼毒水提物小鼠的药物血清对K562细胞增殖的影响

Effects of Serum of Mice Treated With Ruixiang Langdu(Stellera chamaejasme L.) abstract on Proliferation and Clonal Formation of K562 Cells

目的 :研究瑞香狼毒 (SCA )抗癌作用机理。方法 :小鼠SCA灌胃给药后采集血清 ,处理体外培养的人红白血病K562细胞 ,测定其MTT转化及克隆形成率。结果 :SCA小鼠药物血清显著降低K562细胞MTT转化率和克隆形成率 ,并与给药剂量呈正相关 ,给药2h～4h时的药物血清表现出更强的抑制肿瘤细胞增殖作用。结论

OBJECTIVE:To study the antitumor mechanism of Ruixiang Langdu(Stellera chamaejasme L ) abstracts(SCA) METHODS:SCA-contained serum was derived from mice pre-administrated with different oral dosages of SCA and at different times after administration The effects of the serum on the proliferation of K562 leukemic cells were observed with MTT assay and clone formation RESULTS:After mixing with the SCA-contained serum derived at 1,2,4,8h after giving SCA(3,6 and 12g/kg),the rates of MTT transformation and clone formation of K562 cells were decreased significantly The SCA-contained serum 12 h after giving drug was more effective than others CONCLUSION:The SCA-contained serum inhibited the proliferation of tumor cells,which may be one of its important antitumor mechanism