用Iodogen法标记许旺细胞源神经营养因子的实验研究

Labelling Schwann cells derived neurotrophic factor by Iodogen method

目的 用 Iodogen法标记许旺细胞源神经营养因子 (SDNF) ,以便下一步进行 SDNF在组织的受体的研究。 方法 先将 Iodogen涂布于小瓶内 ,加入 SDNF和 Na1 2 5I溶液 ,摇匀 ,在室温下反应 6 min,滴入 Sephadex L H 2 0柱 ,分步收集而成 SDNF放射性碘标记物。 结果 本法可获得的SDNF放射性碘标记物可达 99.7%放化纯度 ,SDNF的神经营养活性和免疫活性没有下降 ,能在 - 80℃保存条件下较稳定 ,符合进行 SDNF受体分析的要求。 结论 用 Iodogen法标记 SDNF方法简单 ,6min内便完成 ,既省时又达到较高的标记率 ,比通常用氯胺 T法容易控制 ,其反应效果好得多。

Objective To label Schwann cells derived neurotrophic factor (SCNF) by Iodogen method for lata study of receptors of SDNF in different tissues Methods SDNF and Na 125 I were incubated in a bottle smeared with Iodogen for 6 min at 25℃ Then the mixture was transferred to a sephadex LH 20 gel column for separafion of 125 I SDNF. Result The radioactivity purity of the 125 I SDNF was 99 7% by paper chromatography The neurotrophic and immuno activity of the 125 I SDNF were not lowered It can be stored in 80℃ condition stably and suitable to the demand of analysing the receaptor of SDNF