带蒂神经植入脊髓后许旺细胞的存活与迁移

The survival and migration of Schwann cells after peripheral nerve grafted into spinal cord

目的 探讨神经组织移植后许旺细胞存活、增殖及诱导轴突再生的能力。 方法 成鼠胸髓损伤后 ,分别移植带血管蒂正中神经 (VN组 )和游离正中神经 (PN组 ) ,术后 8周行组织学检查和形态计量分析。 结果 带血管蒂正中神经组移植神经与脊髓连接紧密 ,有较多新生轴突 ,许旺细胞大量存活和增殖 ,NF、S- 10 0阳性反应均明显高于游离正中神经组。 结论 带血管蒂周围神经移植较接近生理状态 ,许旺细胞存活并向脊髓实质内迁移和大量增殖 。

Objective To compare the survival of Schwann cells and the effects of induce axon regeneration after peripheral nerve grafted into spinal cord Methods A total of 30 adult,female Wistar rats were used in this study,and a 5 mm cord defect of the left lateral column was made at T 1 3 vertebral level The defect was grafted randomly using vascularized (group VN) or free peripheral nerve (group PN) respectively 8 weeks after surgery,the survival and proliferation of Schwann cells were assessed by histological,histomorphometric analysis Results In group VN,peripheral nerve segment was well innervated,plenty of Schwann cells were survived and proliferated In this group,anti NF,S 100 positives fibers were significantly greater than group PN Conclusion The vascularized peripheral nerve could prompt the survival and proliferation of Schwann cells and support the regeneration of central injured axons to certain degrees