帕金森病动物模型的^(99)Tc^m-TRODAT-1显像研究

Imaging of dopamine transporters with ^(99)Tc^m-TRODAT-1,rCBF and MRI in animal model of Parkinson disease

目的 研究帕金森病 (PD)模型猴99Tcm 2 β [N ,N’ 双 (2 巯乙基 )乙撑二胺基 ]甲基 ,3β (4 氯苯基 )托烷 (TRODAT 1)显像与局部脑血流量 (rCBF)及结构改变间的关系 ,探讨99Tcm TRODAT 1显像早期诊断PD的价值。方法 对 5只猴在制成单侧PD模型前后分别进行99Tcm TRODAT 1和rCBF显像 ,并对 2只PD模型猴进行MRI。结果 99Tcm TRODAT 1显像示 ,正常猴 3h纹状体 小脑的放射性比值为 1.48,PD模型猴健侧纹状体 小脑放射性比值为 1 42 ,损毁侧为 0 .96。PD模型猴双侧纹状体血流灌注与正常猴比较无差别 ,MRI未发现形态及结构改变。结论 99Tcm TRODAT 1能特异性地与多巴胺转运体 (DAT)结合 。

Objective To study the relationship between radioactivity distribution and changes of regional cerebral blood flow(rCBF)and tissue structure in the striatum of Parkinson disease (PD) model monkeys with 99Tcm-TRODAT-1 and to estimate the value of imaging with 99Tcm-TRODAT-1 in early diagnosis of PD. Methods 99Tcm-TRODAT-1 and rCBF imaging were performed on five monkeys before and after being made into a single side PD model. Two of the 5 PD model monkeys also received MRI. Results In 99Tcm-TRODAT-1 imaging the radioactivity ratio of striatum to cerebellum (S/C) in the normal monkeys was 1.48 at 180 min after injection of the imaging agent, the ratio of radioactivity in PD model monkeys in their destroyed striatum to that in cerebellum and in normal side striatum were 0.96 and 1.42, respectively. There was no difference in rCBF perfusion between normal and destroyed striatum of the PD model monkeys and between striatum tissue in two hemispheres of the normal monkeys either. The destruction of the tissue structure was not detected in PD model monkeys with MRI. Conclusions 99Tcm-TRODAT-1 can specifically bind dopamine transporters (DAT), sensitively display DAT uptake decrease ahead of the structural damage and cerebral blood flow perfusion decrease in PD model monkeys. It could become a useful imaging modality for the early diagnosis of PD.