期刊文献+

携带毒力岛大肠杆菌性小儿腹泻 被引量:1

Identification of high-pathogenicity island-harboring Escherichia coli from children with diarrhea disease
原文传递
导出
摘要 目的 探讨携带毒力岛大肠杆菌 (HPIEC)在小儿腹泻中的病原学地位。方法 采用PCR扩增和菌落原位杂交检测HPIEC irp2毒力岛基因 ,并用血清学分型和聚合酶链反应 (PCR)法检测各类致泻大肠杆菌。结果 从 10 32例腹泻患儿粪便中分离出 6 5 2株大肠杆菌 ,经血清学和PCR毒力基因分型 ,检出各类致泻大肠杆菌 2 2 5株 ,其中肠致病性大肠杆菌 (EPEC) 2 0株 ,ETEC 81株 ,产志贺样毒素大肠杆菌 (SLTEC) 4 7株 ,产志贺样毒素侵袭性大肠杆菌 (ESIEC) 74株 ,产毒素大肠杆菌 (EIEC)3株。所有致泻大肠杆菌和未能分型的普通大肠杆菌株 ,再用HPIEC irp2探针作菌落原位杂交 ,共检出携带毒力岛大肠杆菌 (HPIEC irp2 ) 112株 ,总阳性率为 17 2 %。其中 41株是从致泻大肠杆菌ESIEC(2 4/ 74)和SLTEC(17/ 47)中检出。携带毒力岛大肠杆菌性小儿腹泻的主要临床表现为食欲不振(87 5 % )、腹痛 (5 8 0 % )、腹泻 (>6次 /d ,75 9% )、发热 (5 0 9% )、以粘液便为主 (6 9 6 % )。结论 携带毒力岛大肠杆菌是引起小儿腹泻的重要病原菌之一。 Objective To investigate the etiological status of high pathogenicity island harboring Escherichia coli (HPIEC) in diarrhea diseases among children. Methods Escherichia coli was Isolated and identified from the stool specimens of 1 032 children with diarrhea diseases by culturing, serotyping, PCR, and colony hybridization. Results 652 strains of E.coli were isolated from the 1 032 stool specimens and confirmed by colony hybridization out of which 225 were identified as diarrheagenic E.coli , including 20 EPEC, 81 ETEC, 47 SLTEC, 74 ESIEC, and 3 EIEC strans. 112 (17.2%) irp2 virulent gene positive E.coli strains were detected from the 652 strains, out of which 24 were from the 74 ESIEC strans and 17 from the 47 SLTEC stranis. The typical clinical symptoms of diarrhea caused by HPI haboring E.coli included anorexia (87.5%), abdominal pain (58.0%), diarrhea (75.9%, over 6 episodes, mostly of mucous stool a day, 75.9%), and fever (50.9%). Conclusion High pathogenicity island harboring E.coli is one of the important pathogens of diarrhea diseases in children.
出处 《中华医学杂志》 CAS CSCD 北大核心 2001年第23期1427-1429,共3页 National Medical Journal of China
基金 山西省科委科学基金资助项目 ( 9810 0 1) 卫生部科学研究基金资助项目 (医学微生物学 96 1 0 2 9)
关键词 大肠杆菌 小儿腹泻 毒力岛 致病岛 临床特征 Diarrhea Escherichia coli Virulence
  • 相关文献

参考文献11

二级参考文献9

  • 1徐建国,程伯鲲,吴艳萍,黄力保,邓庆东,赖心河,刘秉阳,罗兴祖,李惠芬.产志贺样毒素且具侵袭力的大肠杆菌的研究[J].中华流行病学杂志,1994,15(6):333-338. 被引量:54
  • 2吴艳萍,中华流行病学杂志,1994年,15卷,14页
  • 3徐建国,中华流行病学杂志,1994年,15卷,9页
  • 4朱庆义,中华流行病学杂志,1993年,14卷,228页
  • 5朱庆义,中华医学检验杂志,1987年,10卷,232页
  • 6Lai X H,FEMS Microbiol Lett,1999年,172卷,1期,29页
  • 7Guo Xujian,Microbiol Immunol,1996年,40卷,2期,88页
  • 8Guo Xujian,Keusch and Masanobu Kawakami,1996年,321页
  • 9徐建国.毒力岛和细菌毒力的进化[J].中华微生物学和免疫学杂志,1999,19(2):169-171. 被引量:47

共引文献467

同被引文献15

  • 1Buchrieser C,Rusniok C,Frangeul L,et al.The 102-kilobase pgm locus of Yersinia pestis:sequence analysis and comparison of selected regions among different Yersinia pestis and Yersinia pseudotuberculosis strains[ J].Infect Immun,1999,67:4851-4861.
  • 2Clermont O,Bonacorsi S,Bingen E.The Yersinia high-pathogenicity island is highly predominant in virulence-associated phylogenetic groups of Escherichia coli[J].FEMS Microbiol Lett,2001,196:153-157.
  • 3Guyer D M,Kao J S,Mobley H L.Genomic analysis of a pathogenicity island in uropathogenic Escherichia coli CFT073:distribution of homologous sequences among isolates from patients with pyelonephritis,cystitis,and Catheter-associated bacteriuria and from fecal samples[J].Infect Immun,1998,66:4411-4417.
  • 4Hacker J,Kaper J B.Pathogenicity islands and the evolution of microbes[J].Annu Rev Microbiol,2000,54:641-679.
  • 5Hensel M.Salmonella pathogenicity island 2[J].Mol Microbiol,2000,36:1015-1023.
  • 6Moss J E,Cardozo T J,Zychlinsky A,et al.The selC-associated SHI-2 pathogenicity island of Shigella flexneri[J].Mol Microbiol,1999,33:74-83.
  • 7Nataro J P,Kaper J B.Diarrheagenic Escherichia coli[J].Clin.Microbiol Rev,1998,11:142-201.
  • 8Perna N.T,Burland V,Mau B,et al.Genome sequence of enterohaemorrhagic Escherichia coli O157:H7[J].Nature,2001,409:529-533.
  • 9Schubert S,Cuenca S,Fischer D,et al.High-pathogenicity island of Yersinia pestis in enterobacteriaceae isolated from blood cultures and urine samples:prevalence and functional expression[J].J Infect Dis,2000,182:1268-1271.
  • 10Suarez M,Russmann H.Molecular mechanisms of Salmonella invasion:the type Ⅲ secretion system of the pathogenicity island 1[J].Int Microbiol,1998,1:197-204.

引证文献1

二级引证文献6

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部