肥胖大鼠胰岛素抵抗和leptin抵抗的关系研究

Study on Relationship Between Insulin Resistance and Leptin Resistance in Obese-developing Rats

目的 探讨在肥胖形成的过程中Leptin抵抗和胰岛素抵抗何时出现及其因果关系。方法 选取雄性Wistar大鼠2 0只按体重随机分为基础对照和肥胖 2组 ,喂养 5周。每周末称重 ,采尾血测定血糖、胰岛素和Leptin。 结果 肥胖组体重、血糖、胰岛素和Leptin均高于基础对照组。在肥胖形成的过程中Leptin抵抗的发生先于胰岛素抵抗。肥胖组Leptin抵抗首先出现在第 2周末而胰岛素抵抗出现在第 3周末 ,推测Leptin抵抗是胰岛素抵抗的诱因。结论 肥胖是糖尿病重要的危险因素。

Objective To study on relationship between insulin resistance and leptin resistance in obese-developing rats cause-effect relationship between them.Methods Twenty rats were separated to two groups by random:normal group fed normal diet and obese-developing group fed high fat diet.The animals were fed five weeks.The tail blood was collected at each weekend, which was used to measured blood sugar, insulin and leptin.Results Weight,levels of blood sugar,insulin and leptin in obese-developing group were higher than normal group.Leptin resistance appeared at the second week and the insulin resistance appeared at the third week.The timing of leptin resistance appearance was earlier than that of insulin resistance appearance in obese-developing rats.It suggested that leptin resistance was the cause of insulin resistance.Conclusion Obesity was the impontant risk factor.