酶法提取金针菇多糖

Extracting Flammulina Velutipes Polysaccharide by Enzymes

研究了金针菇多糖提取的最佳工艺条件。单因素实验确定了纤维素酶 ,菠萝蛋白酶提取的最佳工艺条件 ,在此基础上进行了复合酶正交实验的研究 ,结果得出最佳工艺条件是时间 80min ,温度 5 0℃ ,pH值 4.0 ,纤维素酶 1.0 % ,菠萝蛋白酶 1.0 % ,果胶酶 1.0 % .

We researched optimum conditions of flammulina velutipes polysaccharide extraction. Single experiments decided the optimum conditions of extraction flammulina velutipes polysaccharied by celluse, bromelin, pectolytic enzyme. Based on the above, orthogonal experiments were carried out and showed that the optimum conditions were reaction time and temperature:80 min and 50 ℃,ph=4.0, celluse 1.0%,bromelin1.0%, pectolytic 1.0%.