放线共生放线杆菌表面相关物质对成骨样细胞Mc3T3-E1碱性磷酸酶和矿化能力的影响

Effect of surface-associated materials(SAM) from Actinobacillus actinomycetemcomitans(Aa) on ALP and mineralization activities of Mc3T3-E1 cells

目的 :观察放线共生放线杆菌表面相关物质对Mc3T3-E1细胞碱性磷酸酶活性的影响。方法 :酶动力学方法。结果 :此物质明显抑制细胞的碱性磷酸酶活性 ,在 10 0mg/L浓度 7d时与对照组差别显著(P <0 .0 5 )。结论 :此物质在骨吸收过程中起重要作用。

AIM :To observe the effect of SAM on ALP activities of Mc3T3-E1 cells. METHODS:Using Enzyme kinetics assay. RESULTS:SAM at concentration of 100mg/L of Aa inhibited ALP activities of Mc3T3-E1 cells (P<0.05). CONCLUSION:SAM may play an important role during bone resorption.