卡那霉素耳中毒后豚鼠耳蜗细胞凋亡的研究

Apoptosis of Cochlear Cells in Guinea Pigs With Kanamycin Ototoxicity

目的 探讨卡那霉素耳中毒后耳蜗毛细胞有无凋亡。方法 取听力正常豚鼠 12只 ,实验组 6只 ,对照组 6只分别给予 40 0mg·kg-1·d-1卡那霉素和生理盐水肌注 ,连续 10天 ,停药一周后处死。取右侧听泡石腊包埋切片。用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记技术 (TUNEL)检测耳蜗细胞凋亡。结果 实验组中Corti器毛细胞及支持细胞 ,血管纹边缘细胞均有凋亡 ,而螺旋神经节细胞无凋亡 ;对照组均无凋亡。结论 耳蜗细胞凋亡在卡那霉素所致耳蜗损害中起重要作用。

Objective To investigate whether cochlear hair cells occur apoptosis following kanamycin treatment in guinea pigs. Methods It was comprised of two groups of six animals each. Experiment group and control group were subjected to administration of kanamycin,im,400 mg·kg -1 ·d -1 ,and saline respectively for successive 10 days. One week later, animals were sacrificed. Left cochlea and right cochlea were taken for cochlea preparation and cochlea section respectively. Apoptosis was detected by terminal deoxynucleotidyl transferase(TdT)-mediated deoxyuridine triphosphate(d-UTP) nick end-labling(TUNEL) method.Results Apoptosis was present in cochlear hair cells, supporting cells as well as marginal cells of stria vascularis except for the spiral ganglion cells in kanamycin treatment guinea pigs. No apoptosis was detected in control animals. Conclusion Apoptosis of cochlear cells play important role in kanamycin ototoxicity.