筑巢式聚合酶链反应检测急性早幼粒细胞白血病PML/RARαmRNA

Detection of PML/RARαmRNA in Acute Promyelocytic Leukemia by Nested Polymerase Chain Reaction

目的 :观察急性早幼粒细胞白血病 (APL)患者PML RARαmRNA的表达情况。方法 :应用逆转录筑巢式聚合酶链反应(NestedPCR)检测了 3 0例APL患者的PML RARαmRNA。结果 :3 0例APL患者中 2 2例检测到PML RARαmRNA ,其中 16例为L型 ,6例为S型。结论 :NestedPCR是检测APL患者PML

Objective:To observe expression of PML/RARαfusion gene of the patients with acute promyelocytic Leukemia (APL).Methods:The PML/RARαmRNA from 30 patiants with APL were assayed by reverse transcription and polymerase chain reaction(RT PCR) and nested PCR. Results:The PML/RARαmRNA was detected in 22 out of 30 patients with APL.Long(L)type was detected in 16 patients and short (S) type was detected in 6 patients.Conclusion:Nested PCR is a very useful and sensitive method in detecting PML/RARαmRNA of the patients with APL.