碱性成纤维细胞生长因子应用于羟基磷灰石眶内植入物的研究

A study of the effects of basic fibroblast growth factor on fibrovascular ingrowth into hydroxyapatite orbital implants

目的 观察不同浓度的碱性成纤维细胞生长因子 (basicfibroblastgrowthfactor,bFGF)对羟基磷灰石 (hydroxyapatite ,HA)植入物纤维血管化的作用。方法 将 36只新西兰白兔随机分为A、B、C、D 4组 ,每组 9只。各组均采用HA植入物植入兔眼眶。A组为对照组 ,B、C、D组在HA球植入术前将植入物分别浸泡于浓度为 0 5、1 5及 5 0g/L国产重组bFGF溶液中 ,术后B、C、D组术眼分别滴用上述不同浓度bFGF的眼液。各组分别于术后 14、2 8及 42d摘取HA植入物行组织学检查 ,并比较各组不同时间的植入物纤维血管化的情况。结果 术后 14d ,纤维血管从HA植入物外周长入植入物内距离 ,C、D与A、B组比较 ,差异有显著性 (χ2 =9 0 ,P =0 0 2 9)。结论 1 5及 5 0g/L浓度的bFGF液对HA植入物术后早期的纤维血管化具有促进作用。

Objective To investigate the effects of different doses of basic fibroblast growth factor (bFGF) on the fibrovascular ingrowth into hydroxyapatite orbital implants. Methods Thirty six New Zealand albino rabbits were divided into 4 groups, and hydroxyapatite orbital spheres were implanted into their orbits. The implants were pretreated with 0 5, 1 5 and 5 0 g/L bFGF solution respectively in 3 groups, the untreated implants were used in the control group. At the postoperative 2, 4 and 6 weeks the implants were harvested. Observers classified the extent of the fibrovascular ingrowth in a blind manner under light microscopy. Results Histologic studies demonstrated that postoperatively, the rate of vascularization of the implants pretreated with 1 5 and 5 0 g/L bFGF solution was significantly increased at week 2 in comparison with that of the control group. No difference was noted between the group with the implants pretreated with 0 5 g/L bFGF solution and the control group. Conclusion The bFGF 1 5 and 5 0 g/L in concentration may promote the fibrovascular ingrowth into hydroxyapatite orbital implants at week 2 after the implantation.