VEGFR-3表达与口腔癌淋巴道转移的关系

Expression of VEGFR-3 and its correlation with cervical lymph node metastasis in oral cancer

目的 了解血管内皮生长因子 (VEGF C)及血管内皮生长因子受体 3(VEGFR 3)在正常口腔黏膜、白斑及鳞癌等组织中的表达规律。方法 采用免疫组化染色及图像分析方法 ,确定VEGFR 3的表达及定量。结果 VEGFR 3主要表达定位在脉管结构 ,在正常及病变上皮组织中也有少量表达。口腔癌VEGF C表达阳性之VEGFR 3染色脉管数目比阴性增多 ,淋巴结转移比无转移增多 (P <0 .0 1) ;而鳞癌比正常黏膜、白斑增多 (P <0 .0 5 ) ;随临床分期或病理分级增高 ,VEGFR 3染色脉管数目有增多趋势 ,但无统计学差异 (P >0 .0 5 )。结论 VEGF C表达的增高 ,通过其受体VEGFR 3导致了淋巴管的增生 。

Objective To investigated the expression of vascular endothelial growth factor receptor 3 (VEGFR 3) in human oral normal,benign epithelium and squamous cell carcinoma to elucidate its role in lymph node metastasis and tumor progression.Methods The expression of VEGFR 3 protein and its ligand VEGF C was examined and analyzed by using of immunohistochemical staining,light microscope and image analysis technique.Results VEGFR 3 was mainly expressed on the vessel structure,and a few of normal epithelium and cancer cells.VEGFR 3 positive vessel count was significantly higher in the VEGF C positive tumors than in the negative tumors( P < 0.01 ),in the lymph node metastasis group than in the no metastasis group( P < 0.01 ),and in the squmous cell cancinoma group than in the normal mucosa group and leukoplakia group( P < 0.05 ).VEGFR 3 positive vessel count did not correlated with tumor stage and clinical stage.Conclusion VEGF C may induce the proliferation of lymphatic vessels via activation of VEGFR 3.They may play a role in lymphatic metastasis via lymphangiogenesis in human oral carcinoma.