重组质粒pCIA-P在大肠杆菌BL21(DE3)中的表达研究

The expression of recombinant plasmid pCIA-P in E.coli BL21(DE3)

目的 证实重组质粒 pCIA P可编码表达具有变形链球菌S .mutans表面蛋白PAc抗原性的表达产物。方法 在选择合适的原核表达系统后 ,诱导外源基因表达出目的蛋白 ,通过蛋白质电泳技术及免疫印迹法检测表达蛋白 ,以观察 pac基因重要抗原决定簇编码区DNA片段在原核细胞中的表达情况。结果 克隆片段在原核系统下可完成表达 ,表达产物保存了S .mutans表面抗原的免疫原性。

Objective To make sure that recombinant plasmid pCIA P could express the products with antigenicity of PAc protein of S.mutans . Methods After choosing an appropriate protokaryotic expression system, the cloned encoding gene of surface protein of S.mutans could be induced and expressed to form desired protein. With SDS PAGE and Western blotting, the expressed protein was detected.Results The cloned DNA was expressed within protokaryotic expression system and the products had the same antigenicity of PAc protein.Conclusion The research result gives an experimental evidence for finding a way of conveniently and rapidly detecting and analysing the expressed products of recombinant DNA vaccine.