bcl-2、Bax基因在面神经损伤后面神经元的表达及对神经元凋亡的调节

Expression of bcl-2 and Bax Genes in Facial Neurons and Their Regulation of Neuron Apoptosis Following Facial Neurotmesis in Rats

目的 :探讨切断面神经干后面神经元bcl_2、Bax基因表达变化与面神经元细胞凋亡的关系。方法 :将 6 0只成年Wistar大白鼠左侧面神经干切断作为实验组 ,右侧面神经干未切断作为对照组。应用ABC免疫组化染色方法和原位末端标记法 (TUNEL)观察术后 3、7、15、2 1、30、6 0d实验组与对照组面神经元内bcl_2、Bax基因的表达及面神经元凋亡的情况。结果 :切断面神经干后 3d ,bcl_2、Bax基因表达开始增多 ,2 1d达高峰 (P <0 0 1) ,bcl_2 /Bax比值达最低 (P <0 0 1) ;切断面神经干后 3d ,实验组面神经元凋亡较对照组增多 ,2 1d达凋亡高峰 (P <0 0 1)。结论 :bcl_2。

Objective: The aim of this study is to investigate the relationship between the expression changes of bcl_2 and Bax genes and their functions in facial neuronal apoptosis after amputating the facial nerve.Methods: Rats were randomized into two groups, including the experimental and the control group. The changes of expression of bcl_2 and Bax genes in the facial neurons and facial neuronal apoptosis were observed after the facial nerve amputation using the methods of ABC immunohistochemical staining and TUNEL.Results: The expression of bcl_2 and Bax genes and the apoptosis of facial neurons were significantly higher in the experimental group than those of the control group from the 3rd to 21st day after amputation, but the bcl_2/Bax significantly decreased compared with that of the control group.Conclusion: This indicated that bcl_2 and Bax might play an important regulation role in the process of facial neurons apoptosis following facial nerve amputation.\;