奶瓶龋的变链菌和乳杆菌水平及产酸分析

Relationship between Streptococcus mutans, Lactobacilus spp. and Lactate-producing Level and Nursing Bottle Caries

目的 :探讨变链菌和乳杆菌与奶瓶龋的关系。方法 :将 2～ 2 5岁幼儿 30名分为有龋组和无龋组 ,每组 15名。从上前牙唇面刮取牙菌斑 ,进行细菌培养 ,对变链菌和乳杆菌数目作出统计。取全口牙菌斑 ,用酶联仪进行乳酸分析。结果 :奶瓶龋儿童有较高的变链菌和乳杆菌检出率 ,两者检出率均为 10 0 %。龋损部位的变链菌和乳杆菌数目为 5 10± 1 36和 4 82± 1 18,超过无龋儿童的相应部位。有龋儿童耐酸菌产乳酸量 136 2 9 16nmol/OD± 16 1 94nmol/OD高于无龋儿童。结论

Objective: The study aimed to investigate the levels of Streptococcus mutans and Lactobacillus spp. as well as the relationship between lactate_productive and aciduric organisms and nursing bottle caries.Methods: Totally, 30 children of 2_ to 2.5_year_old were divided into two groups, including the group of nursing bottle caries and the group of caries_free. Streptococcus mutans and Lactobacillus spp. were isolated from dental plaque of all the children. The amount of lactate produced was measured with a microlitre plate reader. Results: The isolation frequency of Streptococcus mutans and Lactobacillus spp. were both 100 percent in the children with nursing bottle caries, and that the composition of these bacteria from nursing bottle caries lesions was higher than that of caries_free dental surface. Lactate_producing level was higher in the caries group than that of the caries_free group. Conclusion: This study suggests Streptococcus mutans and Lactobacillus spp. may be the major pathogenic bacteria leading to nursing bottle caries.\;