XW630促进去势大鼠骨组织成骨作用的实验研究

Effects of XW630 on Mechanical Properties and Trabecular Structure Parameters of Bone Tissue in Ovariectomized Rats

目的 :探讨抗骨质疏松新药XW6 30在去势大鼠骨组织成骨中的作用。方法 :3月龄SD雌性大鼠 36只 ,随机分为 4组 ,即Sham组、OVX组、OVX加雌酚酮组和OVX加XW6 30组 ,每组 3只。术后 30、6 0、90d各处死 1组动物 ,取两侧胫骨作HE和SEM组织学观察 ,取 1侧股骨作三点弯曲力学强度测试。结果 :HE和SEM观察发现 ,OVX组胫骨干骺端术后 30d即呈骨丢失状态 ,并持续至整个观察期。应用雌酚酮和XW6 30治疗后 ,干骺端骨小梁出现不同程度的骨修复现象 ,整个观察期内XW6 30组成骨活动明显较雌酚酮治疗组活跃。整个观察期内 ,OVX组股骨三点弯曲强度呈下降趋势 ,与Sham组存在显著性差异 (P <0 0 1) ,两个治疗组骨强度则呈上升趋势 ,与OVX组存在显著性差异 (P <0 0 5和P <0 0 1)。结论 :XW6 30能有效地促进去势大鼠骨组织的成骨和预防骨质疏松性骨折的发生。

Objective: The study aimed at investigating the effects of a new anti_osteoporotic drug, XW630 on promoting the osteogenic activity of bone tissue in ovariectomized rats.Methods: Thirty_six female SD rats, three months old, were randomly divided into four groups: sham_operated group (sham), the ovariectomized group (OVX), the esterone_therapy group (OVX+CFT) and the XW630_therapy group (OVX+XW630). Three rats in each group were killed on the 30th, 60th, and 90th day after the operation. The femur of one side was taken for the three_point bending resistance test, and the bilateral tibias were taken for the HE stain and scanning electronic microscopy (SEM) examination.Results: The HE and SEM results indicated that the bone loss appeared in the OVX group 30 days after the operation, which lasted for the whole observing period. While after application of esterone and XW630, there were different degrees of bone repairing around the trabeculae, and the osteogenic activity was obviously active in the XW630 group than that in the esterone group. During the experimental period, the three_point bending resistance of the OVX group gradually decreased and there was a significant difference compared with that of the sham group (P<0.01), while the three_point bending resistance of the two therapy group gradually increased and there was significant difference between the OVX group and the XW630 group (P<0.05 and P<0.01).Conclusion: XW630 can effectively promote osteogenic action and prevent osteoporostic fracture in ovariectomized rats.\;