FHIT基因异常与消化道恶性肿瘤

FHIT alteration in human esophageal, gastric and colorectal carcinomas

目的 探讨消化道恶性肿瘤细胞中三联脆组 (fragilehistidinetriad ,FHIT)基因的异常转录情况。方法 应用巢式逆转录聚合酶链反应 (nestedRT PCR)对 96例消化道恶性肿瘤 ( 2 1例食管癌、4 3例胃癌和 3 2例结肠癌 )及相应的癌旁组织 ,18例正常组织FHIT基因cDNA片断进行扩增。结果 在3 3 .3 %的食管癌组织 ,5 1.2 %的胃癌组织和 3 1.3 %的结肠癌组织中检出异常FHIT转录 ,而在相应的食管、胃、结肠癌旁组织中FHIT异常转录检出率分别为 4 .8%、2 0 .9%和 9.4 % ,二者差异有显著性 (P <0 .0 5 ) ;18例正常组织中未见FHIT异常转录。结论 食管癌、胃癌和结肠癌组织FHIT基因的异常转录可能与消化道恶性肿瘤的发生、发展有关。

Objective To investigate the status of fragile histidine triad (FHIT) gene in human esophageal, gastric and colorectal carcinomas. Methods Ninety six samples of digestive tract cancer (including 21 esophageal carcinomas, 43 gastric carcinomas and 32 colorectal carcinomas) tissues and their adjacent non carcinoma tissues and 18 samples of normal tissue were examined by nested RT PCR for FHIT gene alteration. Results Aberrant transcripts were observed in 33.3% esophageal cancers, 51.7% gastric cancers and 31.3% colorectal cancers. In the adjacent esophageal,gastric and colorectal non carcinoma tissues the rate of aberrant transcripts were 4.8%,20.9% and 9.4%, respectively ( P <0.05). No aberrant transcripts were detected in 18 normal tissues. Conclusion The aberrant transcript of FHIT gene might relate to the occurrence and development of digestive tract carcinomas.