二化螟乙酰胆碱受体α亚基的基因克隆与序列分析(英文)

Cloning and Sequence of Nicotinic Acetylcholine Receptor α Subunit from Chilo suppressalis

烟碱型乙酰胆碱受体 (nAChR)在昆虫的兴奋性突触传递中起着重要的作用 ,同时也是杀虫剂作用的重要靶标。近年来 ,二化螟对作用于昆虫nAChR的沙蚕毒素类杀虫剂杀虫单产生了高抗性。为了研究可能存在的靶标不敏感机制 ,我们采用RT PCR技术 ,对二化螟nAChRα亚基全长cDNA进行了分子克隆。序列分析表明 ,这是 1个新的α亚基基因 ,定名为Csα 1。基因全长为 1997个核苷酸 ,包含了 1个开放阅读框 ,编码 1个 5 0 9氨基酸的成熟蛋白和 1个 2 4氨基酸的信号肽。Csα 1与其他昆虫nAChRα亚基之间有 5 2 %～ 94%的同源性 。

Nicotinic acetylcholine receptors (nAChRs) play a significant role in excitatory synaptic transmission in insects and are the target for chloronicotinyl and nereistoxin insecticides.In recent years,Chilo suppressalis,an economically important pest of rice,developed high resistance against monosultap,a nereistoxin insecticide acting on nAChR.In order to reveal the hypothesized target insensitive mechanism,studies on the molecular property of nAChR from Chilo suppressalis are required.In this study,the full length cDNA of nAChR α subunit from this pest was cloned by RT-PCR.Sequence analysis shows that it is a novel nAChR α subunit,which was named as Cs α 1(Genbank accession No.AF418987).It contains 1?997?bp nucleotides and involves an open reading frame (ORF) encoding a mature protein of 509 amino acids excluding a signal peptide of 24 amino acids.The deduced amino acid sequence was 52%-94% identical to the reported insect nAChR genes.