产低温淀粉酶的海洋真菌筛选及研究

SCREENING AND CHARACTERIZATION OF THE PSYCHROPHILIC AMYLASE-PRODUCING FUNGUS

从黄、东海的近海海底泥样中分离到6株产淀粉酶活力较高的丝状真菌,对其中产淀粉酶活力最高的Penicillumsp.FS010441号菌株产酶条件及酶学性质进行了初步研究。该菌最适生长温度为15℃,最高生长温度为40℃,在0℃也可生长,是典型的耐冷菌。对Penicillumsp.FS010441进行固体培养每克干曲酶活达2622U。Penicillumsp.FS010441所产低温淀粉酶最适作用pH值为6.0,最适反应温度为40℃,但在0℃也表现出一定酶活,而在15℃有较强的酶活。该低温淀粉酶在洗涤、食品、饲料。

stains of psychrophilic amylase-producing fungi were isolated from the near sea mud of the Yellow Sea and the East China Sea. One Penicillum sp. denominated FS 010441 with high amylase production was further studied on growth characteristics and the enzyme properties. The optimum and highest growth temperatures for Penicillum sp. FS 010441 are 15 ℃ and 40 ℃, respectively. The optimum temperature for the amylase is 40 ℃ and the optimum pH is 6.0. But even under 0 ℃, the enzyme still shows some activities. It is expected that the cold-adapted amylase could be used in cold washing, food and fodder industry and so on.