脂质体转移因子的制备及其活性研究

Study on Preparation and Its Bioactivity of Transfer Factor Liposomes(TFL)

以转移因子为包裹对象 ,探讨提高脂质体肽类药物的包封率和减缓渗漏率的方法和条件 ,以及制备过程对活性的影响 .采用逆相蒸发法制备、以正交试验设计筛选最佳配方组合 ,制备负电荷脂质体转移因子 .通过巨噬细胞吞噬试验和E -玫瑰花结试验比较不同剂型、不同给药途径对小鼠免疫功能的影响 .结果表明 :负电荷脂质体转移因子包封率和稳定性均比其他文献报道的有较大的提高 ,包封率 5次平均达 43% ,30d渗漏率 12 % ,具有一定的稳定性 ;E -玫瑰花结试验显示 :制备过程对转移因子活性无显著影响 ;巨噬细胞吞噬试验表明 :不论注射或口服脂质体转移因子 ,均有提高机体免疫功能的作用 。

The optimum experimental conditions of preparating transfer factor liposomes were screened out by the orthogonal design method,transfer factor liposomes prepared by reverse evaporation method and the bioactivity of TFL were investigated in mice using erythrocyte rosette formation test and macrophage phagoytosis test.The results showed that TFL with negative charge we prepared has certain physical and chemical stability and the average entrapment efficiency of TFL prepared in 5 times is up to 43% which is higher than that of other literatures reported,the rate of leakage is about 12% in 30?d,The rate of erythrocyte rosette formation is no significant difference between TF abstracted from TFL and TF;The results of the macrophage phagoytosis test suggested that the effect of TFL,however by injection or orally it is administrated,be similar with that of free TF administrated by injection,superior to that of free TF administrated orally in promoting immune function of mice.