万寿菊中叶黄素的分析方法研究

Research of the Analysis Method of Lutein in Tagetes Patula L.

研究了用柱层析法分离和测定万寿菊及其粗、精加工产品中的叶黄素含量。以硅胶为固定相 ,正己烷 丙酮、正己烷 丙酮 乙醇分别作为胡萝卜素及叶黄素的洗脱剂。其测定波长为 4 74nm ,标准偏差低于 0 .0 12 ,RSD低于1.89%。

The column chromatogrphy is used to seperate and determine the content of lutein in Tagetes Patula L.and its roughing article and meticulous article.Silica gel is taken as solid phase, Hexane acetone as the eluting reagent of carotene and Hexane acetone ethanol as the eluting reagent of lutein.The wavelength of the lutein content is 474 nm,standard deviation is lower than 0.012,and RSD is lower than 1.89%.