生化常规质控方法探讨

Internal quality control method for routine biochemistry analysis

目的 探讨自动生化分析仪仪器校准、室内质控、开放试剂选择的有效方法 ,以寻求合理化的工作模式 ,确保常规检验结果的准确性和一致性。方法 采用BoerhingerMannheim(BM)公司自动生化仪的校正品校正日立 7170A ;用伯乐公司的质控血清在日立 7170A上建立的质控系统对本室三台常规生化分析仪进行质量监控 ;分别用三台生化仪测定 5 0份病人的 16项生化指标 ,对结果进行统计处理。结果 除淀粉酶外 ,其余 16项常规生化指标在三台仪器上的分析结果差异无显著性意义 ,BECKMANDeltaCX7仪器上测定的结果明显高于OLYMPUSAU10 0 0和日立 7170A上的测定结果(F =4.95 7,P =0 .0 0 9) ,其均值分别为 10 2U/L、87U/L和 85U/L。结论 科学的选择分析试剂 ,定期进行仪器校正 ,建立统一的室内质控系统 。

Objective To study the valid correct method for reagent selection, calibration of analyzers, and internal quality control protocol. Methods Reagents were selected by comparing with BRENGER Mannheim(BM)reagent used in Hitachi 7170A. Analyzers were calibrated once a week. One internal quality control chart was set up for three analyzers. Specimens were analyzed by the three machines respectively. Results Except for amylase, all the results obtained from the three analyzers were not significant. Conclusion The recommended quality control protocol is useful to obtain comparable results from different automatic biochemistry analyzers.