一种测定组织因子活性的简便方法

A simple method for quantitation of procoagulant activity of tissue factor

目的 建立一种简便的测定组织因子 (TF)活性的方法。方法 采用已商品化的冻干人凝血酶原复合物作为因子Ⅶ、Ⅹ的来源 ,活化的Ⅹa水解底物S2 2 2 2 ,释放对硝基苯胺 ,40 5nm处的吸光度与标准液中的组织凝血活酶含量成良好的双对数关系 (r=0 .998)。结果 该方法的线性范围从10～ 10 5U。低、高值的批内变异系数为 10 .3%、6 .6 % ,低、高值的批间变异系数为 18.1%、10 .2 %。结论 方法简单、灵敏、能定量 。

Objective To develop a quantitative assay method for the determination of tissue factor. Methods Lyophilized prothrombin complex was used as the source of factor Ⅶ,Ⅹ. Synthetic chromogenic substrate (S2222)was hydrolyzed into p nitroaniline. The absorbency of p nitroaniline measured spectrophotometrically at 405 nm is proportional to TF quantity in logarithm ( r =0.998). Results The linear range was from 10 U to 10 5 U. Low and high coefficients of variation were 10.3%, 6.6% within day and 18.1%, 10.2% day to day. Conclusions The method is very simple, sensitive, with good linearity, and applicable to quantitation of TF activity quickly.