哮喘患者白细胞表面分化抗原23的表达与IgE的相关性研究

Correlation between CD23 expression on white cell′s surface and IgE in asthmatic children

目的 研究哮喘的发病机制 ,探讨哮喘患者白细胞表面分化抗原 (CD) 2 3的表达及与血清IgE的相关性。方法 用流式细胞术检测 2 2例典型哮喘、18例咳嗽变异型哮喘、2 0例喘息性支气管炎、2 0例肺炎和 2 5名正常对照者的外周血淋巴细胞CD19阳性、CD2 3阳性、CD2 3和CD19双阳性细胞 (CD19+ 、CD2 3+ 、CD19+ /CD2 3+ )的百分率 ,同时用免疫化学发光法检测血清IgE水平 ,并进行相关性研究。结果 典型哮喘、咳嗽变异型哮喘患者外周血淋巴细胞CD19+ 、CD2 3+ 、CD19+ /CD2 3+ 的百分率和血清IgE分别为 :(13.5 6± 5 .87) %、(2 6 .5 6± 7.6 1) %、(12 .86± 5 .0 1) %、(12 .6 7± 7.5 6 ) μmol/L和 (13 10± 5 .15 ) %、(2 4.6 6± 8.15 ) %、(12 .10± 4.97) %、(9.45± 4.16 ) μmol/L ,明显高于正常对照组 (7 95± 3.6 5 ) %、(14.92± 3.0 2 ) %、(6 .82± 2 .95 ) %、(3.10± 1.6 8) μmol/L和肺炎组 (P <0 .0 1) ,CD19+ 、CD2 3+ 、CD19+ /CD2 3+ 与血清IgE水平呈显著正相关。喘息性支气管Ⅰ组外周血淋巴细胞CD19+ 、CD2 3+ 、CD19+ /CD2 3+ 及血清IgE也显著高于正常对照组和肺炎组及喘息性支气管炎Ⅱ组 ,喘息性支气管炎Ⅱ组的上述指标与正常对照组和肺炎组差异无显著性。结论 淋巴细胞CD2 3的表达?

Objective To study the relations between CD23 expression on peripheral blood B lymphocytes and IgE in asthmatic children and their pathogenesis. Methods CD19 +, CD23 +, CD19 +/CD23 + expressions of peripheral blood lymphocytes were measured by flow cytometry and serum total IgE level by chemiluminescence immunoassay in 22 children with classical asthma, 18 cough variant asthma (CVA), 20 with asthmatiod bronchitis ,20 with pneumonia and 25 healthy controls. The correlations between CD23 expression on peripheral blood B lymphocytes and IgE in serum were analysed. The 20 children with asthmatiod bronchitis were divided into two groups by the scores whether it reached that for diagnosing asthma after ten months: Group Ⅰ (10 cases) and Group Ⅱ (10 cases). Results CD19 +, CD23 +, CD19 +/CD23 + expressions on lymphcytes were significantly elevated in peripherial blood and serum IgE from children with classical asthma [(13.56±5.87)%,(26.56±7.61)%,(12 86±5.01)%, (12.67±7.56) μmol/L respectively] and CVA [(13.10±5.15)% ,(24.66±8.15)% , (12 10±4.97)%, (9.45±4.16) μmol/L respectively] as compared with those in the children with pneumonia and healthy controls [(7.95±3.65)%, (14.92±3.02)% ,(6.82±2.95)% , (3.10±1.68) μmol/L respectively] ( P <0.01). The positive correlation between CD19 +, CD23 +, CD19 +/CD23 + and IgE was presented. All parameters in the asthmatiod bronchities Group Ⅰ were significantly higher than those of patients with pneumonia and controls. There were no differences between the Group Ⅱ and the patients with pneumonia and controls. Conclusions CD23 expression on B lymphocytes plays an important role in the pathogenesis of asthma. The pathogenesis of asthmatoid bronchitis is same as that of asthma . The detection of CD23 expression on B lymphocytes may provide some important information for early diagnosis of asthma in patients with asthmatoid bronchitis.