经皮睾丸精子抽吸术结合卵浆内单精子注射治疗严重男性因素不育的研究

Percutaneous testicular sperm aspiration and intracytoplasmic sperm injection in the treatment of severe male infertility

目的 了解经皮睾丸精子抽吸术 (PTSA)结合卵浆内单精子注射 (ICSI)技术治疗严重男性因素不育的治疗效果。方法 于 1998年 10月 10日至 2 0 0 0年 12月 31日 ,我中心采用PTSA结合ICSI技术治疗 16 2对严重男性因素不育的夫妇 ,短方案超排卵 ,对处于细胞分裂中期 (MⅡ期 )的成熟卵母细胞行ICSI。结果 185个周期共注射 15 17个MⅡ期卵母细胞 ,其中 990个正常受精 ,正常受精率为 6 5 3% ,移植胚胎周期 15 2个 ,5 4例妊娠 ,临床妊娠率为 35 5 %。结论 PTSA结合ICSI技术为严重男性不育症患者提供了一种快速、方便、无痛、有效的治疗方法。

Objective To investigate the efficacy of intracytoplasmic sperm injection (ICSI) combined with percutaneous testicular sperm aspiration (PTSA)in the treatment of severe male infertility. Methods From October 1998 to December 2000, 162 couples were enrolled, and ICSI and PTSA were adopted. Ovarian stimulation was achieved by the short protocol. All metaphase Ⅱ (MⅡ) oocytes were selected for ICSI. Results 1 517 MⅡ oocytes were injected in 185 cycles, 990 fertilized (65.3%), and 152 embryo implanted. 54 couples achieved clinical pregnancy (35.5%). Conclusion PTSA combined with ICSI is a rapid, convenient, painless and effective approach for the treatment of severe male infertility.