转导bax基因治疗实验性鼻咽癌的研究

Effect of transduction bax gene on experimental nasopharyngeal carcinoma

目的 探讨转导bax基因对鼻咽癌细胞株HNE 1和种植瘤的影响。方法 采用Dosper脂质体介导bax基因转导鼻咽癌细胞株后 ,免疫荧光检测转导效果 ,并分别用四甲基偶氮唑盐(thiazilylblue ,MTT)比色法和流式细胞仪检测转导组和对照组细胞株的凋亡情况 ,并用局部注射的方法观察bax基因对鼻咽癌裸鼠种植瘤的作用。结果 鼻咽癌细胞株HNE 1转导bax基因后 4 8h内 ,免疫荧光法证实肿瘤细胞内有瞬时表达 ;转导前、后HNE1细胞株凋亡指数分别为 10 7%、6 9 4 % ,MTT检测显示转导后 36和 4 8h细胞吸光度为 2 0 0 4和 1 90 2。注射bax基因后实验组 4只裸鼠和注射生理盐水的对照组 3只裸鼠种植瘤的直径 ( x±s)分别为 (1 8± 0 6 4 )cm和 (3 0± 0 4 4 )cm。结论 转导bax基因促进了HNE 1细胞株的凋亡 。

Objective To evaluate the effect of transduction bax gene on nasopharyngeal carcinoma (NPC) Methods The transduced bax gene was mediated by Dosper lipidsome to the HNE 1 cell lines, and confirmed by immunofluorescent stain The apoptosis of the cell lines was studied by MTT stain and flow cytometry and the growth of implanted tumor in naked mouse after local injection of bax geneobserved Results Instantaneous expression of bax gene in HNE1 cell lines was testified by immunofluorecent stain after transduction for 48 hours, the apoptotic index were 10 7% and 69 4% for pre and post transduction, respectively MTT stain showed the values of A in 490nm after transduction to be 2 004(36 h) and 1 902(48 h) The diameter of implanted tumor mass was (1 8±0 64)cm and (3 0±0 44)cm in experimental and control groups, respectively Conclusion The transduction bax gene can enhance the apoptosis of HNE1 cell lines and prevent growth of implanted tumor