猪脾来源免疫抑制组分的分离及其化学本质的研究

Fractionation of Immunosuppressive Component Derived from Porcine Spleen and Studies on Its Chemical Essence

采用 p H梯度洗脱法用阴离子交换柱对猪脾来源免疫抑制组分 (SISE- p1)进行了层析分离 ,获得 7个组分 ,分别命名为 P1～ P7。各组分均具有抑制 PHA诱导的人外周血淋巴细胞 (PBL )转化的生物活性 ,其中以 P4、P6和 P7的活性较强。紫外扫描证明各组分均含有多肽和核酸。用 DNA酶、RNA酶和蛋白酶对 SISE- p1进行酶解 ,然后测定生物活性 ,证明 RNA酶可显著破坏其生物活性 ,蛋白酶可一定程度地降低生物活性 ,而 DNA酶几无作用。从而提示 ,RNA可能是 SISE-

SISE p1, an immunosuppressive component in spleen was chromatographed by anion ion exchange column with pH gradient elution and seven fractions, named P1～P7, were obtained. All the fractions had inhibitory activity on PHA induced human peripheral blood lymphocyte (PBL) transformation, among them P4,P6 and P7 had the higher activity. UV spectra showed that all the fractions contained polypeptides and nucleic acids. The digestions of SISE p1 with DNase, RNase and proteases showed that RNase could remarkably destroy the activity, proteases could lower the activity and DNase almost had no effect. It implied that the immunosuppressive constituents might be RNA fragments.