一氧化氮、白细胞浸润对急性局灶性脑缺血再灌流作用的实验研究

Influences of nitric oxide and leukocytes infiltration on acute focal cerebral ischemic reperfusion in rats

目的 观察一氧化氮 (NO)和白细胞浸润对急性局灶性脑缺血再灌流损伤的作用。方法 建立大鼠急性大脑中动脉栓塞 (MCAO)及再灌流模型 ,采用NADPH -d组织化学方法检测大脑皮质神经元内NOS活性 ,利用MPO活性测定白细胞浸润。结果 ①在脑缺血 15min、30min再灌流 4h时 ,NOS、MPO活性均随着脑缺血时间延长而增加 (P<0 0 1) ;②在脑缺血 6 0min再灌流 4h时 ,NOS活性明显降低 (P <0 0 5 ) ,MPO活性显著增加 (P <0 0 5 )。结论 实验结果说明NO与白细胞浸润在急性脑缺血早期共同作用脑缺血 再灌流损伤 ;在急性脑缺血 再灌流后期 。

Objective To investigate the roles of nitric oxide(NO)and leukocytes infiltration(LI) after acute focal cerebral ischemic reperfusion in rats.Methods The rat models of middle cerebral artery occlusion(MCAO) and reperfusion were established.NADPH d histochemical technique was used to investigate the activity of NOS in cortical neurons.The myeloperoxidase(MPO) activity was measured.Results ①There was a siginificant correlation between the increased level of NOS activity and LI in focal cerebral ischemia(15min,30min) and reperfusion(4h)(P<0 01).②There was a siginificant increased level of LI(P<0 05),and decreased level of NOS activity(P<0 05) after 60min of MCAO and 4h of reperfusion.Conclusion The results showed that NO and LI were involoved in the development of acute cerebral ischemic reperfusion in the early stage;but LI was the main factor involved in the later stage of acute cerebral ischemic reperfusion. [