氨基胍对大鼠脑缺血-再灌流损伤后脑微血管通透性的影响

The effect of aminoguanidine on microvascular permeability after cerebral ischemia/reperfusion

目的 研究选择性诱导型一氧化氮合酶 (iNOS)抑制剂氨基胍对脑缺血 -再灌流损伤时脑微血管通透性的作用。方法 通过缺血 6 0min ,再灌流 6 0min ,造成缺血 -再灌流 (I/R)损伤 ,并制成脑窗模型 ,观察氨基胍对脑微循环通透性的影响。结果 脑缺血 -再灌流损伤后脑微循环通透性明显升高 ,并在 110s后血管外荧光物质浓度就显著高于血管内。而运用氨基胍后 ,在 80s后血管外的荧光物质浓度就显著高于血管内。结论 I/R后脑微循环通透性升高 。

Objective To study the effect of inducible nitric oxide synthase (iNOS) inhibitor,aminoguanidine,on microvascular permeability after cerebral ischemia/reperfusion(I/R).Methods Cerebral ischemia was produced by occlusion of both carotid arteries for 60 minutes.Blood flow was restored for 60 minutes by unclamping the carotids and re perfused with blood.Then the contribution of aminoguanidine to microvascular permeability was observed based on cranial window.Results Cerebral I/R increased the permeability of microvascular net work to fluorescein and the concentration was higher significently out side than that in side the blood vessels after 110 seconds.But when aminoguanidine was used,this phenomenon occurred after 80 seconds.Conclusion I/R increased microvascular permeability.A small amount of nitric oxide produced by iNOS activation may help to maintain microvascular permeability in the early stage after I/R.