自发性细菌性腹膜炎腹水中大肠杆菌潜生体增殖方式的研究

Proliferative manners of E. coli cryptic growth cells in ascites from patients with spontaneous bacterial peritonitis

目的 研究自发性细菌性腹膜炎腹水中大肠杆菌潜生体的增殖方式 ,探讨大肠杆菌潜生体对肠道粘膜的侵袭性。方法 在生物波理论的基础上 ,建立大肠杆菌的波动生长模型 ,观察大肠杆菌潜生体在不同pH、温度、湿度、渗透压、抗生素状态下的生长变化。制备肝硬化鼠动物模型观察大肠杆菌潜生体对肠道粘膜的损害。结果 经波动培养后 ,可见肠道潜生体呈现多种生长方式。自发性细菌性腹膜炎腹水中大肠杆菌潜生体形成率和群集率可显著增高。自发性细菌性腹膜炎腹水中潜生体具有较强的繁殖能力运动能力和抗生素耐受能力 ,从而具有较强的侵袭力 ,而且具有一定的逃逸巨噬细胞吞噬的能力。

Objective To study the proliferative manner of E. coli cryptic growth cells (CGC) from the ascites of spontaneous bacterial patients and their invasiveness in the intestinal tract tissue. Methods Based on bio wave theory and bacterial micro cultured technology, bacterial genetic stability test and minimal inhibitory concentration (MIC) were used to observe the biological characteristics of CGC in pH, temperature, humidity, osmotic pressure. The therapeutic effect of antibiotic on CGC and invasiveness were evaluated. Results In the wave motion cultivation, the CGC of E. coli had great development and proliferation. Bacterial genetic stability and aggregation of the intestinal flora were significantly increased. Its tolerance towards antibiotic was increased, and CGC adapt itself to many disadvantage environments, such as in certain pH values and humidity. The invasiveness of the CGC was significantly increased in the intestinal mucosa and the formation of opportunity bacterial infections. Conclusion CGC have stable species genetic, powerful reproduce, motion and nvasiveness ability, and intensive tolerance towards antibiotic. CGC may play an important role in the pathogenesis of spontaneous bacterial peritonitis.