摘要
目的 探讨胸腹水端粒酶活性检测对良、恶性胸腹水鉴别诊断的价值。方法 采用TRAP PCR银染法和TRAP PCR ELISA半定量法对 32例胸腹水端粒酶活性进行检测。结果 恶性胸腹水中端粒酶阳性检出率 40 .0 % ,良性胸腹水端粒酶阳性率 5 .9% ,恶性胸腹水端粒酶活性 (平均OD值 :0 .46 8)明显高于良性胸腹水 (平均OD值 :0 .0 86 )。结论 胸腹水中端粒酶活性测定可作为鉴别诊断恶性胸腹水的辅助手段。
Objective To investigate the differential diagnostic significance of telomerase activity in the malignant and benign cytologic fluid. Methods Telomerase activity was examined by TRAP-ELISA and TRAP-Silver Staining Assay in 32 cases with ascites or pleural fluid. Results Positive rate of telomerase activity detected in malignant group(40.0%)was significantly higher than that detected in benign group(5.9%).Telomerase activity in malignant group(mean OD 0.468)was significantly higher than that detected in benign group(mean OD 0.086). Conclusion Detection of telomerase activity can be a diagnostic method in differential diagnosis of benign and malignant cytologic fluid.
出处
《中国实验诊断学》
2001年第4期181-182,共2页
Chinese Journal of Laboratory Diagnosis