摘要
介绍一种通过细胞分离及体外培养获得大量新生猪胰岛细胞团的方法。新生猪胰腺经 0 .5～ 1mg .ml- 1的V型胶原酶消化 8～ 1 2min ,再经体外培养 7d ,可获大量纯化的胰岛细胞团。同时 ,新生猪胰岛内的B细胞不仅胰岛素分泌功能良好 ,而且其超微结构正常。提示新生猪胰岛细胞可作为异种移植治疗 1型糖尿病重要的供体来源。
A cell isolation and cell culture in vitro method which can produce a mass of new-born pig islet like clusters was introduced. The new born pig pancreas were incubated in V type collagenase (0.5～1.0 mg.ml -1 ) for 8～12 minutes, and then cultured in vitro for 7 days before large amount of highly purified porcine islets were produced. The neonatal pig islets had good insulin secretion and normal sub-cell structure. The results suggest that the neonatal pig islets are probably a fine donor source for clinical xenotransplantation of islets.
出处
《湖南医科大学学报》
CSCD
北大核心
2001年第6期508-510,共3页
Bulletin of Hunan Medical University
基金
国家自然科学基金( 39770 2 33)。
关键词
胰岛
细胞
胰岛素
体外培养
乳猪
islands of langerhans
cells
insulin
culture in vitro
swine, miniature
methed
