基因重组人IL-6工程菌发酵培养的实验研究

Study on fermentation for recombinant bacterial strain of human interleukin-6

目的 优化基因重组人IL 6 (rhIL 6 )工程菌的发酵和表达条件。方法 选择五种不同的细菌培养基研究rhIL 6工程菌的发酵培养和诱导表达靶蛋白的最佳时间 ,并监测重组质粒在工程菌中的遗传稳定性。结果 1 .5×LB培养基收获的生物菌量最大 ,rhIL 6在工程菌中表达率最高 ;42℃诱导 5hrhIL 6表达量最大 ;所构建的重组质粒在工程菌DH5a中传代稳定 ,未见质粒丢失 ,靶蛋白表达未受影响。结论 提示培养基的成分对构建的工程菌生长影响较大 ,应对发酵培养基及诱导表达条件进行优化。

Objective Optimize the condition of the fermentation and inducing expression of recombinant bacterial strain of human interleukin-6. Methods The fermentation for recombinant bacterial strain of human interleukin-6 was studied by five medium and the optimal time of expression of rhIL-6 was selected and the inheritance stability of the recombinant plasmid of rhIL-6 was analyzed.Results [WT10.5,11.BZ]We found the bacterial yield and expression ratio of rhIL-6 in recombinant bacterial strain were largest in 1.5×LB medium,and expression yield of rhIL-6 was greatest after being induced 5 hours in 42℃.The recombinant plasmid was inherited steadily in DH5a,the loss of recombinant plasmid and the influence of expression of rhIL-6 were not found.Conclusion The component of medium can affect the growth of recombinant bacterial strain greatly,and the fermentation medium and the condition of inducing expression of recombinant bacterial strain should be optimized.