膀胱移行细胞癌组织间质微血管密度和增殖细胞核抗原的表达及意义

Clinical and Prognostic Significance of PCNA Expression and Tumor Interstitial Vascular Density in Transitional Cell Bladder Cancer

目的 探讨膀胱移行细胞癌组织中增殖细胞核抗原 (proliferatingcellnuclearantigen ,PCNA)与肿瘤间质微血管密度 (microvesseldensity ,MVD)和肿瘤侵袭力、复发的关系及意义。 方法 应用免疫组化LSAB法检测72例膀胱移行细胞癌PCNA、第Ⅷ因子相关抗原 (VWF∶Ag)的表达 ,并对病人进行随访分析。 结果 PCNA增殖指数与肿瘤间质微血管密度之间存在正相关性 ,两者的表达皆与膀胱移行细胞癌的病理分级显著相关 ,Ⅰ级与Ⅱ级、Ⅱ级与Ⅲ级之间存在显著性差异 (P <0 .0 1)。侵袭性肿瘤明显高于浅表性肿瘤 (P <0 .0 1)。手术 3年后随访复发组明显高于未复发组 (P <0 .0 1)。结论 PCNA的表达为膀胱移行细胞癌的恶性表型 ,且与膀胱肿瘤间质微血管形成有关 ,上述两项指标联合检测对评估膀胱移行细胞癌的预后有重要的意义。

Objective To evaluate the clinical and prognostic significance of proliferating cell nuclear antigen (PCNA) expression and to study tumor interstitial microvessel density (MVD) in transitional cell bladder cancer.Methods The expression of PCNA and MVD in 72 cases of transitional cell bladder cancer were determined with anti-PCNA McAb and anti-VWF by Ag McAb immunohistochemical staining.Results It was shown that the expression of PCNA in bladder cancer was positively correlated with tumor interstitial vascular density.These two indices increased with the escalation of the tumor grade (P<0.01).The PCNA and MVD expressions in recurrent tumors were higher than those without (P<0.01).Conclusion The results suggest that the expression of PCNA is a malignant phenotype in transitional cell bladder cancer and that it is related to the formation of tumor interstitial vascularity.The measurements of PCNA and MVD expressions are of significance in assessing the prognosis of transitional cell bladder cancer.