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Bcl—2基因在儿童髓母细胞瘤中表达的研究

EXPRESSION OF Bel - 2 mRNA IN MEDULLOBLASTOMA IN CHILDREN
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摘要 目的:从凋亡之异常角度探讨儿童髓母细胞发生、发展及预后的生物学机制。方法:以84例获随访的儿童髓母细胞瘤组织(按术后生存期3、5、10年分为A、B、C三组)为研究对象,用原位杂交及免疫组化染色法分别检测Bcl-2mRNA和增殖细胞核抗原(PCNA)的表达,并用31末端标记法做原位细胞凋亡检测。结果:72例(85.7%)表达Bcl-2mRNA,其阳性率为A>B>C;PCNA阳性肿瘤组织细胞密度与肿瘤细胞Bcl-2mRNA表达水平增加,呈正相关,而凋亡肿瘤细胞密度与肿瘤细胞Bcl-2mRNA表达水平呈负相关。结论:儿童髓母细胞瘤中Bcl-2基因高表达可抑制其凋亡,细胞增殖与凋亡失衡可能是儿童髓母细胞瘤发生发展的重要环节。 To study the biological mechanism of progression and prognosis of medulloblastoma in children, based on abnormal apoptosis. Method: The expression of Bel - 2 mRNA, proliferating cell antigen (PCNA) and the apoptosis of medulloblastoma from 84 children with 3,5,10 survival years (group A, B, C), respectively were studied with in situ hybridization and immunohistochemistry, and in situ death detection (Tunel method) . Result: Positive expression rate of Bel - 2mRNA was 85.7%(72 cases) , and its order was group A > B > C among three group. With the increased expression of Bel - 2mRNA, the cell density of positive PCNA increased, but apoptosis decreased in the medulloblastoma. Conclusion: Over ?expression of Bel - 2 mRNA inhibited apoptosis of medulloblastoma in children. The imbalance between cell proliferation and apoptosis maybe a key to formation and progression of the medulloblastoma in children.
出处 《西南国防医药》 CAS 2001年第6期294-296,共3页 Medical Journal of National Defending Forces in Southwest China
关键词 髓母细胞瘤 BCL-2基因 儿童 medulloblastoma, Bel-2, mRNA, expression
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  • 1Thompason CB,Apoptosis in the pathogenesis and treatment of disease.Science,1995; 267(5203):1456~1462
  • 2Adesina AM,Dunn SI,Moore WE,et al.Expression of po/kipi and P53 in medulloblastoma relationship with cell proliferation and survival.Pathol-Res-Prad,2000; 19(14):243~250
  • 3Miralbell R,Lolnay M,Biens,et al.Pediatric medulloblactoma: prognostic value of P53,Bcl-2,Mibl,and microvessel density.J-Neurooncol,1999; 45(2):103~110
  • 4陈礼刚,朴永旭,张伶俐,秦山,曾力,匡永勤,李讯,曾凡俊.儿童髓母细胞瘤的生物学特性[J].第四军医大学学报,2000,21(9):1146-1146. 被引量:6
  • 5Gyure KA,Prayson RA,Estes ML.Extracerebellar prirmitive neuroectodermral tumors: A clinicopathologic study with Bcl-2 and CD99 immumohistochemistry.Ann-Diagn-Pachol,1999;3(5):276~280
  • 6Fulda S,Jeremias I,Pieteh I,et al.KM.Betulinie acid; a new chemotherapeutic agent in the treatment of nearoectodermal tumors.Klin-Padiatr,1999;211(4):319~322
  • 7Bergmann M,pietsch I,Herms J,et al.Medullormyoblastoma: a histogoical,immunohistochemical,ultrastauctaral and molecular genetic study.Acta-Neuropathol-Berl,1998;95(2):205~212
  • 8Sujimoto Y,Croce CM.Analysis of the structure,transcripts,and protein products of Bcl-2,the gene involved in human follicular lymphoma.Proc Natl Acad Sci USA,1986;83:5214~5218
  • 9Webb A,Cunningham D,Cotter F,et al.Bcl-2 antisense therapy in patients with Non-Hodgkin lymphoma Lancet,1997;349:1137

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