皮肤癌p16及PCNA表达的临床病理研究

Clinicopathological Study of p16 and PCNA Expression in Skin Cancers

目的 :研究p16及增殖细胞核抗原 (PCNA)在皮肤癌发病中的作用。方法 :应用免疫组化S P法检测抑癌基因p16蛋白及PCNA在 5 3例皮肤癌组织中的表达 ,并与癌旁组织和正常皮肤组织对比 ,结合皮肤癌的临床病理特征进行分析。结果 :皮肤癌组织中p16蛋白阳性表达率明显低于癌旁组织和正常皮肤组织 (P <0 0 5、P <0 0 1) ;p16蛋白的阳性表达率随着肿瘤浸润深度的加深而下降 (P <0 0 5 ) ;PTNMⅠ、Ⅱ期皮肤癌组织中p16蛋白阳性表达率明显高于PTNMⅢ、Ⅳ期 (P <0 0 5 )。PCNA指数随着肿瘤浸润深度的加深而增高 (P <0 0 5 ) ;PTNMⅢ、Ⅳ期皮肤癌组织中PCNA指数明显高于PT NMⅠ、Ⅱ期 (P <0 0 5 )。p16阴性组PCNA指数明显高于p16阳性组 (P <0 0 5 )。结论 :p16及PCNA表达与皮肤癌临床病理指标有关 ,皮肤癌的发生与演进是多因素作用的结果 ,两者的检测对估计皮肤癌的恶性度。

Objective To explore the p16 gene and PCNA in the genesis and progression in skin cancers.Method p16 gene and PCNA expressions in 53 cases of skin cancers were detected by immunohistochemistry(S P method)technique.comparing with adjacent non tumor tissues and normal tissues.Results Positive rate of p16 protein in skin cancer is significantly lower than that in adjacent non tumor tissues and normal tissues ( P <0 05; P <0 01).Lower positive rate of p16 protein was observed in patients with deeper invasion( P <0 05).The positive rate in PTNMⅠ,Ⅱ stage is significantly higher than that in PTNM Ⅲ,Ⅳ stage( P <0 05).Higher PCNA index was observed in patients with deeper invasion( P <0 05).The PCNA index of PTNMⅢ,Ⅳ stage were higher than those of PTNMⅠ,Ⅱ stage( P <0 05).Conclusions The expression of p16 and PCNA closely correlate with the clinicopathological parameters of skin cancers.The genesis and progression of skin cancers were a multi factor and multi step process.Detection of the above parameters is of some inferential value in predicting biological aggressiveness and clinical outcome of skin cancers and it may guide to make therapeutic decision.