虎杖提取物白藜芦醇对鼠肝癌细胞H22生长及扩增的影响(英文)

Effect of resveratrol derived from Rhizoma polygoni cuspidati on growth and proliferation of hepatocellular carcinoma H22 of Mouse

目的 测定虎杖的有效成分白藜芦醇对体外培养的小鼠肝癌H2 2的抗癌活性。方法 肝癌细胞H2 2调节到 (1× 10 6)·ml-1,将适宜H2 2细胞数 (2× 10 4 /孔 )接种在 96孔培养板上 ,在 5 %CO2 、37℃培养箱中温育 12h后 ,用MTT法测定白藜芦醇对肝癌细胞的生长抑制率 ,并在显微镜和电镜下观察肝癌细胞的变化。结果 白藜芦醇终浓度为 5 .0 μg·ml-1、10 μg·ml-1及 2 0 μg·ml-1,在 8h、12h、2 4h和 48h及白藜芦醇终浓度为 2 .5 μg·ml-1的抑制率 ,较对照组有显著性差异 (P <0 .0 5 )。显微镜下观察经药物处理 48h的肝癌细胞可见细胞膜不规则、肿胀和染色质断裂 ,电镜下可见细胞出现细胞电子密度增加及出现凋亡小体。结论 白藜芦醇对体外培养的肝癌细胞H2 2的生长具有抑制作用 ,它的机制也许是诱导H2 2细胞凋亡。

Objective To study the anti-cancer activity of resveratrol derived from Huzhang on hepatocellular carcinoma cell H22 of mouse (H22 cell) in vitro.Methods The H22 cell line was adjusted to(1×10 6) cells·ml -1 ,and the cells suspension were incubated in 96-well plates(2×10 4)·well -1 for 12h before the addition of resveratrol,and then were incubated CO 2 incubator with 5% CO 2 and 37℃ with resveratrol in different concentrations for different time, the inhibitive rates of H22 cells induced by resvertrol was determined by the MTT assay. The macroscopical and intracellular urtrastructure of H22 cells was investigated by light microscope and scanning electron microscope.Results The inhibiting rates of resveratrol in 5.0*!μg·ml -1 ,10*!μg·ml -1 and 20*!μg·ml -1 at 8h,12h,24h and 48h,and 2.5*!μg·ml -1 at 24h and 48h were more significantly than those of control groups(P<0.05).The inordinace of membrance,swelling and H22 cells treated with resveratrol 5*!μg·ml -1 for 48h showed chromatin fragmentation of cell under miroscopy and increase of electron density,and apearance of apoptotic body under electron microscopy.Conclusions Resveratrol can inhibit the growth and proliferation of mouse hepatocellular carcinoma H22 in vitro whose probable mechanism is induce apoptosis of H22 cells.