乳腺癌中p^(53)基因的表达及与雌激素受体的关系

Relationship between expression of p^(53) gene and estrogen receptor in human breast carcinoma

目的 研究乳腺癌中 p53 基因的表达对乳腺癌生物学行为影响的意义。方法 应用S -P免疫组织化学方法对 5 4例乳腺癌石蜡包埋组织进行检测 ,研究乳腺癌组织中p53 基因的表达率、表达特点与临床病理和雌激素受体 (ER)的关系。结果 在人的乳腺癌中 p53 蛋白阳性染色主要位于细胞核中 ,阳性表达率为 5 5 .6 % ;ER阳性表达率为 46 .3% ;p53 基因阳性表达与乳腺癌组织学分级呈正相关 (P <0 .0 5 ) ;与临床病理分期无关 (P >0 .0 5 ) ;与淋巴结是否发生转移呈正相关 (P <0 .0 5 ) ;与ER受体表达呈负相关 (P <0 .0 5 )。结论 p53 基因突变参与了乳腺癌的发生和发展 ;p53 蛋白抑制了乳腺癌ER的分化。

Objective To study the affect of the expression of p 53 gene on the biological behavior of human breast cancer and the relationship between expression of p 53 protein and estrogen receptor(ER). Methods Using S-P immunohistochemical method,the expression of p 53 in 54 breast cancer tissue specimens fixed paraffin were detected. Meanwhile,the correlation of the expression rate and expression features of p 53 gene to the clinico-pathology and ER were observed.Results The majority of p 53 protein in human breast tumor tissue were located in cell nuclei and the positive rate was 55.6%.The expression rate of ER was 46.3%.Statistic analysis suggested that the positive expression of p 53 gene correlated with histological grade and metastasis (P<0.05),but did not relation with pathological stage (P>0.05).Positive expression of p 53 was negatively correlatiion with ER(P<0.05).Conclusion p 53 gene mutation involved in oncogenesis and development of breast cancer and it might inhibit the ER expression in breast carcinoma.