HFRS患者PBMC和异型淋巴细胞多种活化标志表达的研究

STUDIES ON THE ACTIVATED ANTIGEN EXPRESSION ON THE LYMPHOCYTES AND LYMPHOBLASTS FROM HFRS PATIENTS

应用McAb的APAAP免疫组化技术对肾综合征出血热(HFRS)患者外周血淋巴细胞的Tac(CD25),TLiSA1,TfR(CD71)和Ia四种活化抗原表达进行了研究,结果表明,HFRS患者急性期外周血淋巴细胞的各种活化抗原表达明显高于恢复期,急性期分别为:36.4±9.3％,36.5±8.4％,43.1±14.3％,42.3±8.0％;恢复期分别为20.0±6.6％,18.9±4.6％22.4±8.5％,23.6±3.9％。对Percoll纯化后的异型淋巴细胞和小细胞各种活化抗原表达进行检测的结果提示,异型淋巴细胞中各种活化抗原的表达显著高于小细胞,异型淋巴细胞分别为:48.1±13.0％,52.1±12.9％,55.7±8.7％,52.7±6.7％;小细胞分别为:12.9±2.3％,13.9±2.7％,15.1±2.9％,23.5±2.6％。此结果对于了解患者机体免疫状态及异型淋巴细胞来源和功能有一定的意义。

In the present study, the activated antigen expressions on lymphoblasts and lymphocytes from HFRS patients were investigated with APAAP technique. The results indicated: (1) The positive rate of activated antigen expression on lymphocytes during the acute phase was significantly higher than that of convalescent phase. The Tac, TLiSA1,TfR and Ia positive lymphocytes from acute phase patients were 36.4±9.3%, 36.5 ± 8.4%, 43.1 ±14.3% and42.3±8.0% respectively, and those from convalescent phase patients were 20.0 ±6.6%,18.9± 4.6%,22.4 ± 8. 5%and 23.6 ± 3.9%respectively. (2)Theratio of activated antigen expression on lymphoblasts(Tac: 48.1 ± 13.0%;TLiSAl: 52.1±12.9%, TfR: 55.7±8.1% Ia: 52.7±6.7%) was also higher than that on the small lymphocytes (Tac: 12.9 ± 2.3%, TLiSAl: 13.9±2.7%, TfR: 15.1 ± 2.9, Ia:23.5± 2.6%). These results were of great significance in understanding the immune status as well as origin and function of lymphoblasts in HFRS patients.