荧光寿命测定的现代方法与应用

Fluorescence Lifetime Measurements: Modern Methods and Applications

介绍了时间相关单光子计数、相调制和频闪等三种现代荧光寿命测定方法的工作原理 ,指出了各种方法的优点和局限性 ;介绍了时间相关单光子计数实验数据的处理方法 ;概述了时间分辨荧光技术在化学和生命科学中的应用。

The principles and characteristics of some of the modern techniques, including time\|correlated single\|photon counting (TCSPC),phase modulation and strobe techniques, for fluorescence lifetime measurements have been briefly introduced. The advantages and disadvantages of each method have also been pointed out. The common method used for the analysis of the fluorescence decay, taking TCSPC as an example, has been discussed in detail. On the basis of these introductions, the applications of time\|resolved fluorescence techniques in chemical and biological research have been overviewed.\;